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A novel NIR fluorescent probe based on p-mercaptophenylboronic acid-functionalized copper nanoclusters for capmatinib
Ali O Alqarni1, Rayed Ali A Alqahtani1, Ashraf M Mahmoud1,2
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Najran University, Najran, Kingdom of Saudi Arabia.
Analytical Methods : Advancing Methods and Applications
|March 30, 2026
Summary
A novel fluorescent probe using copper nanoclusters accurately quantifies capmatinib (CAMB) in biological samples. This method offers high sensitivity and selectivity for cancer therapy monitoring.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biomedical Engineering
Background:
- Accurate quantification of capmatinib (CAMB) is crucial for cancer therapy.
- Existing methods may lack sensitivity or selectivity for biological fluid analysis.
Purpose of the Study:
- To develop a sensitive and selective fluorescent probe for CAMB detection.
- To validate the probe's performance in biological samples for clinical applications.
Main Methods:
- Fabrication of p-mercaptophenylboronic acid-functionalized copper nanoclusters (p-MPBA@CuNCs).
- Utilized fluorescence quenching mechanism (IFE and static quenching) for CAMB detection.
- Validated analytical performance according to ICH guidelines and tested in human serum and urine.
Main Results:
- Achieved a wide linear range (0-370 µM) and a low detection limit (1.8 nM).
- Demonstrated rapid response (1.5 min) and excellent selectivity against interferents.
- Obtained high recovery rates (98.6%-104.5%) in human serum and urine samples.
Conclusions:
- The p-MPBA@CuNCs probe provides accurate, precise, and robust quantification of CAMB.
- This system is suitable for real-world clinical sample monitoring in cancer therapy.
- The developed probe represents a significant advancement in pharmacokinetic evaluation and therapeutic drug monitoring.
