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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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Nanofibrous Interface-Engineered Microgel Array Enabling Streamlined Digital PCR.

Serin Kim1,2, Yoon-Ha Jang1, Sang-A Lee1,3

  • 1Center for Advanced Biomolecular Recognition, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.

ACS Sensors
|March 30, 2026
PubMed
Summary
This summary is machine-generated.

A novel microgel array chip simplifies digital PCR (dPCR) partitioning for accessible, rapid nucleic acid quantification. This robust platform offers high accuracy in diagnostics, including for SARS-CoV-2 detection.

Keywords:
PEG microgelsSARS-CoV-2digital PCRmicrogel arrayoxygen inhibition

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Materials Science

Background:

  • Digital PCR (dPCR) offers high sensitivity and absolute quantification but relies on complex, less portable microfluidic systems.
  • Existing dPCR platforms often require specialized equipment like pumps or vacuum systems for sample partitioning.
  • There is a need for more accessible, portable, and user-friendly dPCR technologies for widespread diagnostic applications.

Purpose of the Study:

  • To develop and validate a novel microgel array chip for simplified and rapid digital PCR (dPCR) sample partitioning.
  • To assess the performance of the microgel array dPCR platform in terms of accuracy, speed, and accessibility.
  • To demonstrate the utility of the microgel array dPCR for accurate nucleic acid quantification in clinical samples.

Main Methods:

  • Fabrication of a microgel array chip with 2003 porous microgels (1 nL each) within a 1 cm2 area.
  • Utilizing the microgel's hydrophilic, oil-repelling nanofibrous surface for sequential partitioning of PCR mixture and oil.
  • Evaluating amplification stability under fast thermal cycling and comparing absolute quantification accuracy with RT-qPCR.

Main Results:

  • The microgel array chip enabled simple and rapid partitioning, completing diagnostic assays in under 1 hour.
  • The platform demonstrated high accuracy in absolute nucleic acid quantification, correlating strongly with conventional RT-qPCR.
  • Successful application in detecting SARS-CoV-2 in nasopharyngeal swab samples highlights its diagnostic potential.

Conclusions:

  • The microgel array dPCR platform provides a practical and accessible alternative to conventional dPCR systems.
  • Its design facilitates robust sample partitioning and stable amplification, enabling rapid and accurate molecular diagnostics.
  • This technology holds promise for point-of-care testing and broader implementation of digital PCR.