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Standardized protocol for plasticity assessment in the aging mouse neocortex using choline-chloride perfusion.

Pia Kruse1, Charlotte Schob1,2, Kerstin Schwabe2,3

  • 1Hannover Medical School, Institute of Neuroanatomy and Cell Biology, Hannover, Germany.

Frontiers in Aging Neuroscience
|March 30, 2026
PubMed
Summary

A new protocol enables high-quality brain slice preparation from aged mice for studying synaptic function. This method reveals age-related differences in synaptic plasticity, crucial for understanding neurodegenerative diseases.

Keywords:
acute slicingcLTPcholine-chlorideforskolinperfusionsynaptic plasticity

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Area of Science:

  • Neuroscience
  • Aging Research
  • Cellular Biology

Background:

  • Age-related synaptic dysfunction contributes to neurodegeneration.
  • Preparing high-quality brain tissue from aged animals for analysis is difficult.
  • Existing methods struggle to preserve neuronal structure and function in aging brains.

Purpose of the Study:

  • To develop a standardized protocol for preparing intact acute brain slices from mice of different ages.
  • To enable combined structural and functional analysis of individual neurons across the lifespan.
  • To investigate age-related changes in synaptic transmission and plasticity.

Main Methods:

  • Standardized acute brain slice preparation using transcardial choline-chloride perfusion.
  • Analysis of cortical lamination and subcellular synaptic structure in medial prefrontal cortex (mPFC) pyramidal neurons.
  • Whole-cell patch-clamp recordings to examine spontaneous excitatory synaptic transmission and forskolin-induced chemical long-term potentiation (cLTP).

Main Results:

  • The protocol reliably yields intact cortical slices from young and aged mice.
  • Cortical lamination and synaptic structure are preserved in layer 2/3 pyramidal neurons.
  • Synaptic plasticity, measured by cLTP, shows age-related differences in expression.

Conclusions:

  • The developed protocol is a reproducible framework for studying synaptic transmission and plasticity in the aging cortex.
  • This method is broadly applicable to research on age-related brain disorders and neurodegeneration.
  • The findings highlight age-dependent alterations in synaptic plasticity mechanisms.