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Updated: May 18, 2026
Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens
Published on: April 27, 2011
Integrated metagenomic sequencing and phage display-based immunoprecipitation sequencing for presumptive viral
Jessica G Shantha1,2, Cindi Chen1, Armin Hinterwirth1
1Francis I. Proctor Foundation, University of California, San Francisco, CA, USA.
Purpose:
To report a case of uveitis in which unbiased metagenomic sequencing (MDS) and phage immunoprecipitation sequencing (PhIP-Seq) were performed on intraocular fluid.
Observations:
A female patient with a past medical history of human immunodeficiency virus (HIV-1) who presented with chronic active anterior and intermediate uveitis with cystoid macular edema in the left eye. She had a previous ocular history of viral retinitis presumed to be secondary to cytomegalovirus (CMV). An anterior chamber tap was performed for viral polymerase chain reactions (PCRs), MDS, and pathogen PhIP-Seq. PCR testing and MDS were negative for pathogen genetic materials. PhIP-Seq detected antibody enrichment of CMV, as well as HIV-1 andSARS-CoV-2.
Conclusions:
The combination of MDS and PhIP-Seq has the potential to provide additional insights into the pathogenesis and the ocular microenvironment of uveitis patients.
