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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Mar 31, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Programmable, target-induced fluorogenic CRISPR-tDeg platform for live-cell RNA visualization.

Huimin Zhong^1,2, Jiayan Zhou^1,2, Fujun Qin²

¹College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

Nucleic Acids Research

|March 30, 2026

Summary

This summary is machine-generated.

We developed CtDeg, a CRISPR-based RNA imaging platform that links fluorescence to target RNA binding and uses degradation to reduce background signals. This enables precise visualization of RNA dynamics and interactions in living cells.

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Last Updated: Mar 31, 2026

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Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage CoTrAC

Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage CoTrAC

Published on: March 15, 2013

Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

RNA imaging in living cells is limited by low specificity and high background fluorescence.
Existing CRISPR-based RNA imaging systems struggle with constitutive signal emission and non-specific binding.

Purpose of the Study:

To develop a novel RNA imaging platform with enhanced specificity and reduced background noise.
To enable precise visualization of RNA localization, dynamics, and function in living cells.

Main Methods:

Engineered a CRISPR-dCas13-tDeg system (CtDeg) with a Pepper RNA motif for target-induced fluorescence.
Optimized degron (tDeg) variants to maximize signal-to-noise ratio.
Applied CtDeg to visualize paraspeckle assembly, SARS-CoV-2 RNA transport, and virus-induced lncRNA accumulation.

Main Results:

CtDeg demonstrated significantly lower background and higher specificity compared to conventional methods.
Real-time imaging of paraspeckle assembly and SARS-CoV-2 genomic RNA transport was achieved.
CtDeg provided the first direct imaging evidence of virus-induced NEAT1_2 lncRNA accumulation, revealing host-virus interactions.

Conclusions:

CtDeg is a versatile, target-induced RNA imaging platform compatible with multiple Cas13 orthologs and fluorescent proteins.
This platform offers broad applications in synthetic biology and RNA biology for probing RNA behavior in living cells.