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Updated: Mar 31, 2026

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

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Published on: August 22, 2025

Image Analysis Methodologies.

Ashwin Kumar Rajagopalan¹, Daniel Biri²

¹Department of Chemical Engineering, The University of Manchester, Manchester, UK. a.rajagopalan@manchester.ac.uk.

Advances in Biochemical Engineering/Biotechnology

|March 30, 2026

Summary

This summary is machine-generated.

This chapter details established practices for characterizing biomolecular crystals using imaging techniques. It covers tools, methods, and challenges for effective crystal population analysis in research and industry.

Keywords:

Crystallization Image Analysis Imaging Machine Learning Particle Size Distribution

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Area of Science:

Biochemistry and structural biology
Crystallography
Imaging science

Background:

Accurate characterization of biomolecular crystals is crucial for understanding crystallization processes.
Existing measurement tools for crystal analysis have varying advantages and limitations.
Standardized practices are needed for reliable crystal population characterization.

Purpose of the Study:

To compile established practices for characterizing biomolecular crystal populations using image-based systems.
To provide a comprehensive overview of imaging techniques for crystal analysis.
To equip practitioners with knowledge for applying these methods in research and industry.

Main Methods:

Introduction and comparison of available measurement tools for crystal characterization.
Detailed description of image acquisition, data analysis, and visualization steps.
Discussion of current challenges and limitations of imaging tools.

Main Results:

Established practices for image-based biomolecular crystal characterization are compiled.
A comparative analysis of measurement tools is presented.
Considerations for each step of image acquisition to data visualization are offered.

Conclusions:

Image-based systems offer valuable methods for characterizing biomolecular crystals.
Understanding the steps and limitations of imaging tools is essential for effective application.
This compilation aims to enhance the application of crystal characterization techniques in scientific and industrial settings.