Related Experiment Video
Updated: Apr 1, 2026

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement
Published on: February 22, 2022
Project-based learning for aerobic capacity and key components of distance running performance
John J Durocher1,2, Deepanshi Dahiya1,2
1Department of Biological Sciences, Purdue University Northwest, Hammond, Indiana, United States.
None:
It can be difficult for some students to comprehend maximal aerobic capacity (V̇o2max) and endurance performance. We present a project-based learning example used in our advanced human physiology course. This project is based largely on a seminal paper but utilizes chapters from the course textbook and additional peer-reviewed studies. After we cover background information on topics such as muscle physiology, metabolic pathways, expired air analysis, V̇o2max, and lactate threshold, students join one of three groups to prepare and deliver presentations. Those topics and groups are based on 1) history of V̇o2max, 2) rate limiters of V̇o2max, and 3) additional determinants of endurance performance. The instructor covered topics from the textbook on sports physiology including oxygen uptake at the onset, during, and after exercise. The course instructor also provided a brief review of sport-specific V̇o2max and lactate threshold testing in hockey players. Students in the class agreed to hold one class period in the laboratory for direct use of expired air analysis. The lab emphasized the relationship between intensity and carbohydrate versus fat utilization. During the project presentations in the final week of class, group 1 summarized key points such as the use of a Douglas bag and the difference between V̇o2max and V̇o2peak, while group 2 summarized central (e.g., cardiac output) versus peripheral (e.g., muscle mitochondria) limitations, and group 3 summarized other key factors for endurance performance such as lactate threshold and running economy. This project-based assignment was an excellent way for students to better comprehend V̇o2max and endurance performance.NEW & NOTEWORTHY This project-based assignment provided students a unique way to comprehend human metabolism, cardiorespiratory and muscle physiology, and applied exercise science concepts such as maximal aerobic capacity (V̇o2max), lactate threshold, and running economy. Topics covered during this assignment are important for health, performance, and longevity. Students were excellent at working in small groups, basing presentations on peer-reviewed literature, and applying their communication skills. Students strongly agreed with "teaching methods" and scored well on final exam questions related to the project.
Related Concept Videos
Exercise and Muscle Performance
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...
Exercise and Cardiovascular Response
Light to moderate physical activity initiates a series of interconnected responses in the body. The heart rate modestly increases in anticipation of the workout, followed by widespread vasodilation as oxygen consumption by skeletal muscles increases. This results in decreased peripheral resistance, increased capillary blood flow, and accelerated...
Exercise and Cardiac Output
Sustained exercise increases the muscles' oxygen demand, which can be...
Lung Capacity
Respiratory Capacities
One key metric is the Inspiratory Capacity (IC), which represents the maximum amount of air that can be inhaled with full effort. IC is calculated by summing the tidal volume and inspiratory reserve volume, typically ranging from 2.4 to 3.6 liters.
The Functional Residual Capacity (FRC) represents the air in the...

