Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

109
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
109
Ion Exchange01:17

Ion Exchange

1.5K
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oral connexin43-peptide-loaded milk extracellular vesicles mitigate lethal radiation injury while preserving tumour radiosensitivity.

Cancer letters·2026
Same author

Designing polymer-peptide conjugates to target dipeptide repeat aggregates implicated in amyotrophic lateral sclerosis.

Journal of materials chemistry. B·2026
Same author

Peptide stereocomplex cross-links for polymer hydrogels.

Chemical science·2025
Same author

Orally Delivered Milk-Derived Nanovesicles Loaded with Connexin 43 Peptides for Targeted Cardiac Ischemia-Reperfusion Therapy.

bioRxiv : the preprint server for biology·2025
Same author

Presenting Antimicrobial Peptides on Poly(ethylene glycol): Star-Shaped vs Comb-Like Architectures.

Macromolecules·2025
Same author

Expanding the Design Space of Polymer-Metal Organic Framework (MOF) Gels by Understanding Polymer-MOF Interactions.

Chemistry of materials : a publication of the American Chemical Society·2024

Related Experiment Video

Updated: Apr 1, 2026

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

11.3K

Reversible, Polymeric Complexation of Therapeutic Peptides Using Esterification.

Aditi S Gourishankar1, Mark S Bannon1, Kelly M Bukovic1

  • 1Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22903, United States.

ACS Macro Letters
|March 30, 2026
PubMed
Summary

Esterifying therapeutic peptides reversibly enhances their charge and hydrophobicity, enabling tunable complexation with polymers. This approach allows for controlled peptide release, offering new possibilities for drug delivery systems.

More Related Videos

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
06:15

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

8.2K
Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.9K

Related Experiment Videos

Last Updated: Apr 1, 2026

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

11.3K
Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
06:15

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

8.2K
Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.9K

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery

Background:

  • Polyelectrolyte complexes enable tunable, aqueous complexation of therapeutic peptides.
  • Existing methods rely on electrostatic and hydrophobic interactions, limiting complexation with weakly charged, hydrophilic peptides.
  • Permanent modifications to peptides can hinder activity and release.

Purpose of the Study:

  • To investigate reversible peptide-polymer complexation using esterification.
  • To explore the role of reversible charge and hydrophobicity in complexation.
  • To demonstrate tunable complexation and release of therapeutic peptides.

Main Methods:

  • Reversible esterification of the therapeutic peptide α-carboxyl terminus 11 (αCT11).
  • Complexation with anionic poly(methacrylic acid).
  • Hydrolysis of ester bonds to trigger peptide release.
  • Modification of polymer hydrophobicity.

Main Results:

  • Esterification reversibly increased peptide charge and hydrophobicity, enabling complexation.
  • Hydrolysis of ester bonds led to peptide dissociation over 24 hours.
  • Hydrophobic aspartimide intermediates promoted aggregation and prolonged complexation.
  • Reduced polymer hydrophobicity abrogated complexation, highlighting its importance.

Conclusions:

  • Esterification is a viable strategy for reversible polymeric complexation of weakly charged, hydrophilic peptides.
  • Polymer hydrophobicity can be tuned to control reversible complexation for specific delivery needs.
  • This method offers promising prospects for advanced peptide-based drug delivery systems.