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Cell Probe Cocktail Enables Ratiometric Mirna Detection With Enhanced Sensitivity And An Extended Dynamic Range.

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Cell Probe Cocktail Enables Ratiometric Mirna Detection With Enhanced Sensitivity And An Extended Dynamic Range.

Related Experiment Video

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Cell Probe Cocktail Enables Ratiometric miRNA Detection with Enhanced Sensitivity and an Extended Dynamic Range.

Shengkai Zhang¹, Lanshuang Hu¹, Luyin Wang¹

¹Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, P. R. China.

Analytical Chemistry

|March 30, 2026

View abstract on PubMed

Summary

This summary is machine-generated.

This study introduces a novel cell probe cocktail for sensitive and wide-range microRNA (miRNA) detection. The method enhances sensitivity and expands the dynamic range for disease monitoring.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

Biotechnology
Molecular Diagnostics
Nanotechnology

Background:

Sensitive and wide-range microRNA (miRNA) detection is vital for early disease diagnosis and monitoring.
Existing platforms face challenges in simultaneously achieving high sensitivity and a broad dynamic range due to inherent trade-offs.

Purpose of the Study:

To develop a novel cell probe cocktail system for ratiometric miRNA detection.
To overcome the sensitivity-dynamic range trade-off in miRNA sensing platforms.

Main Methods:

Integration of nicking endonuclease-assisted strand displacement amplification (SDA) with a cell probe cocktail.
Construction of counting probes using aptamer-functionalized magnetic nanoparticles conjugated to cells.
Leveraging DNA programmability for target recognition-induced assembly/disassembly of cell probes.

Main Results:

Demonstrated ratiometric detection of miRNA-21 over a wide concentration range (0.2-100 nM).
Achieved a low limit of detection (LOD) of 0.038 nM for miRNA-21.
Successfully enhanced sensitivity and expanded the dynamic range concurrently.

Conclusions:

The developed cell probe cocktail strategy offers a versatile platform for sensitive and wide-dynamic-range miRNA detection.
DNA programmability enables adaptive sensing for diverse DNA-based architectures.
This approach holds promise for improved disease monitoring through advanced miRNA diagnostics.