Three-dimensional organoid screening using a Leica Thunder microscope

3D organoids are vital for biomedical research, offering complex tissue models superior to 2D cultures for studying development, disease, and drug responses. High-throughput imaging of these structures is challenging. This report details a method for screening 3D organoids using the Leica Thunder microscope, featuring Computational Clearing for enhanced deep imaging. The protocol covers sample preparation, optimized image acquisition using LAS X, and analysis with FiJi/ImageJ for quantification. Applications include drug discovery and disease modeling. The Leica Thunder facilitates efficient 3D organoid screening.