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The Transcription Factor OsWRKY64 Interacts With OsART1 to Positively Regulate Al Resistance in Rice.
Changzhao Chen1,2, Qiang Zhang3,4, Yonggang Xu5
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
Plant Biotechnology Journal
|March 31, 2026
Summary
A newly identified protein, OsWRKY64, enhances aluminium tolerance in rice by stabilizing the key transcription factor OsART1. This interaction boosts OsART1
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- Rice (Oryza sativa) possesses significant aluminium (Al) tolerance.
- The C2H2-transcription factor OsART1 is vital for regulating Al tolerance in rice.
- The posttranscriptional regulation of OsART1 is not well understood.
Purpose of the Study:
- To identify and characterize OsWRKY64 and its role in Al tolerance.
- To elucidate the interaction between OsWRKY64 and OsART1.
- To understand the regulatory mechanism of Al tolerance in rice.
Main Methods:
- Yeast two-hybrid assays to detect protein-protein interactions.
- Subcellular localization studies.
- Quantitative real-time PCR and Western blotting to analyze gene and protein expression.
- Phenotypic analysis of mutant and overexpression lines under Al stress.
Main Results:
- OsWRKY64 interacts with OsART1 and is localized in the nucleus.
- OsWRKY64 expression is induced by Al, particularly in roots.
- OsWRKY64 enhances OsART1 stability and transcriptional activity, leading to increased Al resistance.
- OsART1 upregulates OsWRKY64 expression, forming a negative feedback loop.
Conclusions:
- OsWRKY64 is a crucial positive regulator of Al tolerance in rice.
- OsWRKY64 functions by stabilizing OsART1 and enhancing its activity.
- A negative feedback loop exists between OsART1 and OsWRKY64, contributing to Al tolerance regulation.
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