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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Apr 1, 2026

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Aptamer- and Ribozyme-Engineered sgRNAs for Conditional Control of CRISPR/Cas9 Function.

Wei Wang1, Liting Li2, Xiaohui Li2

  • 1Central Laboratory, The Second People's Hospital of Foshan (Affiliated Foshan Hospital of Guangdong Pharmaceutical University), 528000 Foshan, Guangdong, China.

Frontiers in Bioscience (Landmark Edition)
|March 31, 2026
PubMed
Summary

Engineered CRISPR/Cas9 systems with responsive sgRNAs offer precise control for gene editing and imaging. These advancements in synthetic biology hold promise for disease modeling and therapeutics.

Keywords:
CRISPR-Cas systemsRNA aptamersgene expression regulationgenetic engineeringguide RNAribozymes

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Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Genetics

Background:

  • The CRISPR/Cas9 system is a powerful tool for genome editing and regulation.
  • Synthetic biology enables engineering of CRISPR/Cas9 for conditional responses.
  • Single guide RNA (sgRNA) modifications are key to this engineering.

Purpose of the Study:

  • To review condition-responsive CRISPR/Cas9 systems.
  • To discuss design strategies and applications.
  • To highlight potential in synthetic biology and therapeutics.

Main Methods:

  • Integrating functional nucleic acid elements (aptamers, ribozymes, aptazymes) into sgRNA.
  • Engineering sgRNA for responsiveness to molecular signals.
  • Developing systems for spatiotemporal control.

Main Results:

  • Created CRISPR/Cas9 systems responsive to small molecules, proteins, and metabolites.
  • Achieved conditional gene editing, activation, repression, and imaging.
  • Demonstrated applicability in prokaryotic and eukaryotic cells.

Conclusions:

  • Condition-responsive CRISPR/Cas9 systems offer advanced control in synthetic biology.
  • These systems have significant potential for disease modeling and therapeutic development.
  • Future work should focus on improving specificity, efficiency, and applicability.