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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

49
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
49

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Related Experiment Video

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Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
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A Miniaturized Enzymatic Lactate Sensor for Continuous Monitoring in Oxygen-Depleted Tissue Microenvironments.

Caitlyn X Chen, Vishal Venkatesh, Eiko Nakamaru-Ogiso

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    This study developed an implantable microsensor for direct, real-time tissue lactate measurement. It accurately tracks metabolic changes even in low-oxygen disease environments, overcoming wearable sensor limitations.

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    Area of Science:

    • Biomedical Engineering
    • Biosensors
    • Metabolic Monitoring

    Background:

    • Real-time tissue lactate measurement is crucial for metabolic disorder diagnosis and monitoring.
    • Traditional wearable lactate sensors lack tissue-specific information and diagnostic accuracy.
    • Dynamic physiological environments and oxygen depletion pose challenges for sensor operation.

    Purpose of the Study:

    • To develop an implantable microsensor for direct, continuous, real-time lactate measurement within target tissues.
    • To assess the sensor's performance in oxygen-deficient microenvironments.
    • To overcome limitations of existing non-invasive monitoring systems.

    Main Methods:

    • Fabrication of a miniaturized enzymatic lactate sensor (0.7x1.0x0.12 mm) on gold electrodes.
    • Immobilization of lactate oxidase with biochemical modifications for enzyme stability.
    • Use of a permselective membrane for analyte diffusion regulation and prolonged operational lifetime.
    • Electrochemical evaluation of sensor performance across various lactate concentrations.

    Main Results:

    • The sensor demonstrated an expanded linear response range (LRR) from 0.2 to 50 mM.
    • Achieved a rapid response time of 8 seconds, even in oxygen-deficient conditions.
    • Validated performance in simulated biological environments and across pathophysiological conditions.

    Conclusions:

    • The developed sensor enables continuous, implantable monitoring of tissue lactate levels.
    • It overcomes limitations of traditional systems in capturing local metabolic activity under reduced oxygen availability.
    • Provides a platform for real-time metabolic assessment supporting diagnostics and therapeutic monitoring.