Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

10.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
10.1K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

1.1K
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1.1K
Stringent Response in E. coli01:23

Stringent Response in E. coli

468
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
468
Cell Signaling in Plants01:25

Cell Signaling in Plants

7.1K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
7.1K
Types of RNA01:23

Types of RNA

73.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.9K
Translational Regulation01:29

Translational Regulation

862
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
862

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD2AP is a potential prognostic biomarker of renal clear cell carcinoma.

Cancer medicine·2024
Same author

Influence of Aging on Outcomes of Sacubitril/Valsartan in Hypertensive Patients with Heart Failure: A Multicenter Retrospective Study.

Anatolian journal of cardiology·2024
Same author

Abnormal Lower Limb Biomechanics During a Bilateral Vertical Jump Despite the Symmetry in Single-Leg Vertical Hop Height in Athletes After ACL Reconstruction.

Orthopaedic journal of sports medicine·2024
Same author

Allo-HSCT with TBI-based preconditioning for hepatosplenic T-cell lymphoma: two case reports and systematic review of literature.

Frontiers in oncology·2024
Same author

GPX4 is a potential diagnostic and therapeutic biomarker associated with diffuse large B lymphoma cell proliferation and B cell immune infiltration.

Heliyon·2024
Same author

[Correlation between methylation of interferon regulatory factor 6 gene promoter in renal tissues and overall survival of patients with Kidney renal clear cell carcinoma].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2024

Related Experiment Video

Updated: Apr 2, 2026

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.7K

TaGR-RBP, a Glycine-rich RNA-binding Protein in Wheat, Activates Rust Resistance Through ROS Burst.

Mengying He1,2, Shan Zhang1, Yurong Yan1

  • 1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling 712100, China.

Journal of Agricultural and Food Chemistry
|March 31, 2026
PubMed
Summary

Glycine-rich RNA-binding proteins (GR-RBPs) are crucial for plant stress responses. This study shows TaGR-RBP positively regulates wheat immunity against Puccinia striiformis (Pst) by binding RNA and inducing defense responses.

Keywords:
Puccinia striiformis f. sp. tritici (Pst)disease resistanceglycine-rich RNA-binding proteinwheat

More Related Videos

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

15.2K

Related Experiment Videos

Last Updated: Apr 2, 2026

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.7K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K
Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

15.2K

Area of Science:

  • Plant Molecular Biology
  • Plant Pathology
  • Wheat Genetics

Background:

  • Glycine-rich RNA-binding proteins (GR-RBPs) regulate plant stress responses.
  • The role of GR-RBPs in wheat immunity against Puccinia striiformis (Pst) is largely unknown.

Purpose of the Study:

  • Identify GR-RBP genes in wheat.
  • Characterize the function of TaGR-RBP in wheat's immune response to Pst.

Main Methods:

  • Gene identification and expression analysis.
  • Subcellular localization, Virus-induced gene silencing (VIGS), and CRISPR-Cas9 gene editing.
  • Reactive Oxygen Species (ROS) detection, RNA-binding assays (REMSA), and AlphaFold3 prediction.

Main Results:

  • 58 GR-RBP genes were identified in wheat.
  • TaGR-RBP expression is upregulated during wheat-Pst interactions and it localizes to the nucleus and cytoplasm.
  • Silencing or knocking out TaGR-RBP compromises wheat resistance to Pst, reducing H2O2 accumulation and increasing fungal proliferation.

Conclusions:

  • TaGR-RBP plays a positive regulatory role in wheat immunity against Pst.
  • TaGR-RBP exhibits RNA-binding activity and induces ROS accumulation.
  • Findings provide a basis for engineering disease-resistant wheat and developing sustainable disease control strategies.