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Updated: Apr 2, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Nitrospira-Driven partial Denitrification: Kinetics, Mechanisms, and synergistic potential with anammox
Jiaying Wang1, Ju Huang2, Deyong Li1
1School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.
None:
In nitrite-oxidizing bacteria (NOB)-enriched biomass, formate (3 mM) promoted partial denitrification (PD) by NOB-Nitrospira, resulting in a nitrite accumulation rate of 1.02 ± 0.17 mg-N/(g-SS·h). The relationship between nitrite accumulation rate and formate concentration followed Michaelis-Menten kinetics (R2 = 0.95), with a half-saturation constant of 3.18 mM. The Nitrospira in NOB biomass harbored fdh gene (encoding formate dehydrogenase) and nitrate reduction genes narG and napA, which were upregulated by 137.0%, 5.5%, and 127.0% with formate addition, respectively. This revealed a synergy between formate oxidation (electron donation) via fdh and nitrate reduction, thereby enabling PD. At 3 mM formate, the addition of NOB into anaerobic ammonium oxidizing bacteria (AnAOB) biomass increased the nitrogen removal rate from 1.74 to 3.56 mg-N/(g-SS·h), demonstrating effective coupling between NOB-driven PD and anammox. This study reveals that NOB Nitrospira can shift from a competitor to a collaborator with AnAOB, offering a potential new pathway for nitrogen removal.
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