Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.6K
pre-mRNA Processing02:01

pre-mRNA Processing

58.4K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
58.4K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

17.5K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
17.5K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-specific regulation of angiogenin in Alzheimer's disease.

Molecular psychiatry·2026
Same author

ModiCal: A Targeted Calibration Workflow for Site-Specific m<sup>5</sup>C Validation by Nanopore Direct RNA Sequencing.

ACS chemical biology·2026
Same author

Real-time transcriptomic profiling in distinct experimental conditions.

eLife·2026
Same author

ADAR1 and ADAR2 associate with the RNA exosome and modulate RNA stability.

Nucleic acids research·2026
Same author

Cannabinoid CB<sub>1</sub> receptors drive oligodendrocyte maturation during myelin regeneration.

Cell communication and signaling : CCS·2026
Same author

Yeast elongation factor homolog New1 protects a subset of mRNAs from degradation by no-go decay.

Nucleic acids research·2026
Same journal

Demonstration of a quantum C-NOT gate in a time-multiplexed fully reconfigurable photonic processor.

Nature communications·2026
Same journal

Nonlinear quantum light source with van der Waals ferroelectric NbOX<sub>2</sub> (X = Br, I).

Nature communications·2026
Same journal

Antagonistic histone H2A variants and autonomous heterochromatin formation shape epigenomic patterns in Arabidopsis.

Nature communications·2026
Same journal

The long tail of nitrate pollution in groundwater challenges governance of global water quality.

Nature communications·2026
Same journal

Select microbial metabolites promote tau aggregation in a murine tauopathy model.

Nature communications·2026
Same journal

Warming climate has lengthened global intense tropical cyclone seasons.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Apr 2, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.3K

Mapping human pre-rRNA processing and modification at single nucleotide resolution using long read nanopore

Stefan Pastore1, Ludivine Wacheul2, Lioba Lehmann3

  • 1Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg-University Mainz, Mainz, Germany.

Nature Communications
|March 31, 2026
PubMed
Summary
This summary is machine-generated.

NanoRibolyzer, a nanopore sequencing method, precisely identifies and quantifies precursor rRNA (pre-rRNA) molecules and their modifications. This advance offers new insights into ribosome biogenesis and RNA processing pathways.

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

12.3K

Related Experiment Videos

Last Updated: Apr 2, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.3K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.9K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

12.3K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Ribosome biogenesis is crucial for cell function, involving complex precursor rRNA (pre-rRNA) processing.
  • Traditional methods for studying pre-rRNA processing lack the resolution needed for detailed analysis.

Purpose of the Study:

  • To introduce NanoRibolyzer, a novel nanopore-based long-read sequencing approach.
  • To enable ab initio identification, quantification, and modification mapping of pre-rRNAs.
  • To provide high-resolution insights into rRNA processing and modification dynamics.

Main Methods:

  • Nanopore-based long-read sequencing (NanoRibolyzer).
  • Supervised and unsupervised mapping for pre-rRNA identification.
  • Cell fractionation for spatial deconvolution of processing.
  • Analysis of exoRNase activity and RNA modification mapping (pseudouridine).

Main Results:

  • Detection of known and novel pre-rRNAs with single-nucleotide resolution.
  • Delineation of rRNA cleavage events and spatial separation of nuclear/cytoplasmic processing.
  • Characterization of exoRNase activity and identification of condition-specific processing fingerprints.
  • Pseudouridine mapping revealed extensive modification of primary transcripts and hypomodification of aberrant intermediates.

Conclusions:

  • NanoRibolyzer significantly advances the study of ribosome biogenesis by providing unprecedented resolution.
  • The method reveals novel insights into rRNA processing pathways, RNA modifications, and the functions of ribosome assembly factors.
  • NanoRibolyzer has potential for biomarker discovery in RNA processing defects.