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Related Experiment Video

Updated: Apr 2, 2026

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Published on: December 29, 2023

Assays for Plasma ADAMTS13 Activity and Inhibitors Using Recombinant FRETS-VWF73 Peptide.

Sarah H Gralnek¹, Szumam Liu¹, X Long Zheng²

¹Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kansas City, KS, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 31, 2026

Summary

This summary is machine-generated.

A new assay, FRETS-rVWF73, can quickly measure ADAMTS13 activity, which is crucial for diagnosing thrombotic thrombocytopenic purpura (TTP). This method aids in understanding TTP and developing new treatments.

Keywords:

ADAMTS13 Activity Autoantibodies Inhibitor Thrombotic thrombocytopenic purpura

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Last Updated: Apr 2, 2026

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

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Published on: December 29, 2023

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Published on: August 14, 2017

Area of Science:

Biochemistry
Hematology
Molecular Biology

Background:

Thrombotic thrombocytopenic purpura (TTP) is a critical condition caused by low ADAMTS13 enzyme activity.
ADAMTS13 normally prevents blood clots by cleaving ultra-large von Willebrand factor (ULVWF) multimers.
ADAMTS13 deficiency can be inherited or acquired through autoantibodies.

Purpose of the Study:

To develop a simple, rapid, and cost-effective assay for measuring ADAMTS13 activity and inhibition.
To facilitate the diagnosis of TTP and advance translational research on ADAMTS13.

Main Methods:

The study details the FRETS-rVWF73 assay, including substrate expression, purification, and labeling.
A comprehensive protocol for the assay is provided, along with potential challenges and solutions.
The assay is designed to quantify ADAMTS13 enzymatic activity and inhibition.

Main Results:

The FRETS-rVWF73 assay provides a means to measure ADAMTS13 activity.
The assay is suitable for quantifying inhibition of ADAMTS13 activity.
This assay can be applied in various biological contexts for research purposes.

Conclusions:

The FRETS-rVWF73 assay is a valuable tool for assessing ADAMTS13 activity and inhibition.
This assay supports TTP diagnosis and translational research into ADAMTS13-related disorders.
The described method offers a practical approach for studying ADAMTS13 function.