Simultaneous Determination of ABA, IAA, SA, and JA by UPLC-ESI-MS/MS

This protocol describes a method for the simultaneous extraction and quantification of the main phytohormones involved in plant stress responses and development-abscisic acid (ABA), indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid (SA)-from small quantities of plant tissue. It combines minimal extraction and purification steps with high-throughput analysis by ultra-high pressure liquid chromatography coupled with tandem mass spectrometry (UPLC-ESI-MS/MS). Surrogate standards are used for accurate quantification, making the method suitable for diverse plant matrices and absolute quantification.