Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

13.8K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Cell Atlases as Meta-Analytic Compasses for Developmental Biology: A Case Study Using the Arabidopsis Root.

Journal of experimental botany·2026
Same author

Cell-specific dedifferentiation in <i>Arabidopsis thaliana</i>.

bioRxiv : the preprint server for biology·2026
Same author

From binding to networks: methods for identifying transcription factor targets in plant systems.

Frontiers in plant science·2026
Same author

Glow with the Flow: Reproducible Analysis of Transiently Transformed Protoplasts Using Dual Fluorescent Reporters in R.

bioRxiv : the preprint server for biology·2025
Same author

Engineered Yeasts Displaying PETase and MHETase as Whole-Cell Biocatalysts for the Degradation of Polyethylene Terephthalate (PET).

ACS synthetic biology·2025
Same author

Core biological principles and tools stemming from basic Arabidopsis research.

The Plant cell·2025

Related Experiment Video

Updated: Apr 2, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

482

Automated Flow-Cytometric Readout of Reporter-Gene Activation in Transiently Transformed Protoplasts.

Samuel Chisholm1, Joseph S Taylor2, R Clay Wright3

  • 1Department of Biological Systems Engineering, Polytechnic Institute and State University, Blacksburg, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2026
PubMed
Summary

This study introduces a high-throughput method using Arabidopsis protoplasts for analyzing reporter-gene activity. This approach enhances the speed and accuracy of dissecting gene regulatory mechanisms.

Keywords:
Automated analysisFlow cytometryProtoplastsReporter-gene activation

More Related Videos

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.1K
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

13.5K

Related Experiment Videos

Last Updated: Apr 2, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

482
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.1K
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

13.5K

Area of Science:

  • Plant Molecular Biology
  • Functional Genomics
  • Gene Regulation

Background:

  • Reporter-gene assays are crucial for understanding gene regulation but often lack throughput and quantification.
  • Traditional whole-plant assays are time-consuming and difficult to scale for large-scale studies.

Purpose of the Study:

  • To develop a streamlined, high-throughput method for analyzing reporter-gene activity.
  • To enable rapid and robust quantification of transcriptional responses in plant cells.
  • To provide a versatile platform for functional genomics research.

Main Methods:

  • Utilizing transiently transformed Arabidopsis thaliana mesophyll protoplasts.
  • Employing the pBeaconRFP vector for positive-fluorescent selection and flow cytometry/cell sorting.
  • Introducing the pEvTV Gateway-compatible vector for flexible construct delivery.
  • Implementing an automated pipeline for reproducible flow-cytometric data analysis.

Main Results:

  • Demonstrated rapid, robust, and scalable quantification of transcriptional responses.
  • Successfully isolated transformed cells using flow cytometry and fluorescence-activated cell sorting.
  • Validated the method by analyzing DR5 auxin-responsive reporter activation via gain-of-function Auxin Response Factor expression.

Conclusions:

  • The developed protocol offers a versatile and efficient platform for high-throughput functional genomics.
  • The methods are adaptable to various plant tissues and species.
  • This approach significantly improves the analysis of reporter-gene activity for dissecting gene regulatory mechanisms.