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Updated: Apr 3, 2026
06:47
Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
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Conventional and novel platelet parameters: Clinical and laboratory approaches
Alireza Bayani1, Zahra Mohammadi1, Paul Harrison2
1Division of Laboratory Hematology and Blood Banking, Department of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
Summary
Advanced platelet counting methods and indices offer promising diagnostic and prognostic value for various disorders. Standardization and AI integration are crucial for wider clinical adoption and improved reliability.
Area of Science:
- Hematology
- Clinical Pathology
Background:
- Complete blood count (CBC) platelet indices are vital for diagnosing and monitoring hematologic and systemic disorders.
- Methodological variability and lack of standardized protocols can impact diagnostic reliability.
Purpose of the Study:
- To review conventional and advanced platelet counting methods.
- To evaluate the analytical strengths and limitations of these methods.
- To discuss the diagnostic and prognostic value of platelet indices like MPV, PDW, P-LCR, IPF, and PCT.
Main Methods:
- A narrative literature review was performed.
- Searches included PubMed, Scopus, Web of Science, and Google Scholar up to June 1, 2025.
Main Results:
- Modern analyzers use impedance, optical scatter, fluorescence, and hybrid methods for platelet enumeration.
- Advanced fluorescence and hybrid methods improve accuracy, especially in thrombocytopenia.
- Platelet indices show potential in conditions like ITP, MDS, ACS, and sepsis, but analyzer variability and lack of standards are challenges.
Conclusions:
- Advanced platelet counting methods and indices hold diagnostic and prognostic promise.
- Further validation and standardization are needed for clinical implementation.
- Artificial intelligence may enhance accuracy, reproducibility, and clinical utility.
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