Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Centrioles and Centrosomes

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly.

Science advances·2025

Same author

Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomes.

Science advances·2025

Same author

Humanoid robots to mechanically stress human cells grown in soft bioreactors.

Communications engineering·2024

Same author

Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.

Nature communications·2024

Same author

Regulation of centrosome size by the cell-cycle oscillator in Drosophila embryos.

The EMBO journal·2024

Same author

Mob4 is essential for spermatogenesis in Drosophila melanogaster.

Genetics·2023

Search research articles

Related Experiment Video

Updated: Apr 3, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Radial Profile-Based Quantification of Centrosomal Proteins.

¹Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.

|April 2, 2026

Summary

This study introduces a new method to measure protein levels within centrosomes in fruit fly embryos. This technique uses radial profile analysis for precise quantification of centrosomal protein abundance and distribution.

Keywords:

3D-SIM Centriole Centrosome dynamics Drosophila embryo FRAP Pericentriolar material Quantitative fluorescence imaging Radial profile analysis Super-resolution microscopy

More Related Videos

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Imaging Centrosomes in Fly Testes

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Related Experiment Videos

Last Updated: Apr 3, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Imaging Centrosomes in Fly Testes

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Area of Science:

Cell Biology
Microscopy Techniques

Background:

Centrosomes are vital organelles for cell division and cilia formation.
Understanding centrosome protein dynamics is crucial for cell biology research.

Purpose of the Study:

To present a protocol for quantifying relative centrosomal protein abundance in Drosophila melanogaster embryos.
To enable high-resolution, quantitative analysis of dynamic centrosome assembly.

Main Methods:

The protocol involves embryo collection, dechorionation, live imaging, and confocal microscopy.
Radial profile analysis of fluorescence intensity is used to quantify protein abundance and spatial distribution.
Integration with fluorescence recovery after photobleaching (FRAP) and 3D structured illumination microscopy (3D-SIM) is described.

Main Results:

Radial profiling provides quantitative measurements of centrosomal protein abundance and spatial distribution.
The method allows for the generation of relative or normalized intensity profiles.
Compatibility with both confocal and super-resolution microscopy is demonstrated.

Conclusions:

This protocol offers a robust method for quantitative analysis of centrosome assembly in a genetically tractable system.
Combining radial profiling with advanced imaging techniques enhances the resolution and scope of centrosome research.