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MicroRNA differential expression analysis in canine visceral hemangiosarcoma formalin-fixed, paraffin-embedded
Laura Machado Ribas1,2, Kerstin Muner2,3, Nelly Elshafie3
1Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, United States.
Frontiers in Veterinary Science
|April 2, 2026
Summary
This study identified microRNA (miRNA) signatures as potential biomarkers for canine visceral hemangiosarcoma (HSA). These findings suggest miRNAs could aid in diagnosing this aggressive cancer, even in archived tissues.
Area of Science:
- Veterinary Oncology
- Molecular Diagnostics
- Biomarker Discovery
Background:
- Canine visceral hemangiosarcoma (HSA) is an aggressive cancer with limited diagnostic options.
- MicroRNAs (miRNAs) show promise as stable, disease-specific biomarkers.
Purpose of the Study:
- Identify miRNA biomarkers for canine splenic and cardiac HSA.
- Validate miRNA expression in formalin-fixed, paraffin-embedded (FFPE) tissues.
Main Methods:
- Small RNA sequencing and RT-qPCR were used to analyze miRNA expression.
- Tissues from dogs with HSA and non-neoplastic controls were examined.
Main Results:
- 67 and 71 differentially expressed miRNAs were found in splenic and cardiac HSA, respectively.
- 13 miRNAs were validated in each tissue type, showing concordance with sequencing.
- Validated miRNAs are involved in key oncogenic signaling pathways.
Conclusions:
- miRNA signatures may offer diagnostic value for canine visceral HSA.
- Validated miRNAs can be detected in archived FFPE tissues.
- Potential for future liquid biopsy applications exists.
Keywords:
angiosarcomacancer biomarkersmiRNAspathway analysissmall RNA sequencingvisceral hemangiosarcoma
