Related Experiment Video
Updated: Apr 3, 2026
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Detection of reactive oxygen species associated with photodynamic therapy
Ming-Chen Xiong1, Li-Ya Niu1, Kun-Xu Teng1
1College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China. kunxuteng@bnu.edu.cn.
Abstract:
Accurate detection of reactive oxygen species (ROS) associated with photodynamic therapy (PDT) is essential for evaluating the performance of photosensitizers and elucidating PDT mechanisms. This review systematically evaluates the detection of ROS related to PDT using commercially available probes, including singlet oxygen, superoxide anion radicals, hydroxyl radicals, hydrogen peroxide, and peroxynitrite. We compare their structures, detection mechanisms, performance, advantages, and limitations, and offer practical guidance to help researchers select appropriate ROS detection strategies for PDT studies.
More Related Videos
05:16Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
Published on: January 19, 2024
14:25Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
Published on: May 13, 2022