'1347B' module confers durable stripe rust resistance in wheat

The stripe rust resistance molecular module '1347B', comprising Yr9-1RS/1BL , Yr30-3BS , YrZH22-4BL , and YrZH84-7BL , could provide stable and durable stripe rust resistance without a yield penalty in wheat breeding program. Wheat stripe rust is a significant epidemiological disease that severely impacts wheat production. The aggregation of multiple stripe rust resistance genes constitutes a pivotal breeding strategy for reducing pathogen evolution of virulence and enhancing the resistance level in wheat. Investigating the resistance conferred by various combinations of stripe rust resistance genes and assessing their impact on wheat yield are essential for understanding the synergistic regulatory mechanisms of these genes on host immunity and yield. In this study, 254 F₇ recombinant inbred lines (RILs) derived from the cross ZK331/ZK32 were evaluated for stripe rust severity at the adult-plant stage and thousand-kernel weight (TKW) across six environments over a two-year period. Through QTL mapping and molecular marker detection, four stripe rust resistance genes, Yr9 (1RS/1BL), Yr30 (3BS), YrZH22 (4BL), and YrZH84 (7BL) were identified in the RIL population. The results demonstrated that pyramiding these genes conferred a high level of adult plant stage resistance to stripe rust. Analysis of agronomic traits, including TKW, plant height, spike length, and kernel number per spike, in RIL population with different combinations of these genes revealed no adverse effects under rust-free conditions and a highly significant positive effect under stripe rust infection. The molecular module '1347B', comprising Yr9-1RS/1BL , Yr30-3BS , YrZH22-4BL , and YrZH84-7BL , provided stripe rust resistance across multiple growth stages without a yield penalty. This study elucidates the resistance efficacy and yield stability provided by the '1347B' module, providing a valuable genetic resource and theoretical foundation for designing high-yielding wheat varieties with enhanced stripe rust resistance.