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Related Experiment Video

Updated: Apr 3, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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Research progress on nucleic acid amplification-based detection technologies for phytopathogenic fungi.

Baoyu Wang1, Mei Liu1, Hui Wang1

  • 1Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

Applied Microbiology and Biotechnology
|April 2, 2026
PubMed
Summary

Rapid molecular detection technologies, including nucleic acid amplification and CRISPR/Cas systems, are essential for identifying phytopathogenic fungi. These advanced methods aid in early diagnosis and precision management of fungal diseases, significantly reducing agricultural losses.

Keywords:
Nucleic acid amplificationNucleic acid extractionPhytopathogenic fungiRapid detection

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Plant Pathology

Background:

  • Phytopathogenic fungi cause significant global crop losses, exceeding 30% annually.
  • Accurate identification of fungal pathogens is critical for effective disease management and prevention.
  • Molecular detection methods offer rapid and sensitive tools for pathogen identification.

Purpose of the Study:

  • To review advancements in nucleic acid-based detection techniques for phytopathogenic fungi.
  • To evaluate the strengths, limitations, and applicability of various molecular diagnostic platforms.
  • To highlight the role of these technologies in precision agriculture and disease management.

Main Methods:

  • Review of thermal cycling-based methods (PCR, qPCR, ddPCR).
  • Examination of isothermal amplification platforms (LAMP, RPA).
  • Analysis of CRISPR/Cas-assisted assays combined with isothermal amplification (RPA-CRISPR, LAMP-CRISPR).

Main Results:

  • Nucleic acid amplification technologies provide rapid and sensitive detection of fungal pathogens.
  • Isothermal amplification and CRISPR/Cas platforms enable field-deployable diagnostics with minimal instrumentation.
  • Integrated workflows facilitate early disease diagnosis and precision management strategies.

Conclusions:

  • Molecular detection technologies are crucial for combating agricultural losses due to fungal diseases.
  • Emerging technologies like isothermal amplification and CRISPR/Cas offer promising solutions for on-site diagnostics.
  • These advancements support integrated strategies for early detection and precise management of plant fungal diseases.