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An Integrated Electrochemical Biosensor Array Enabling Extracellular Vesicles Disruption and Multiplexed Lung Cancer

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    We developed a portable biosensor for rapid lung cancer biomarker detection in extracellular vesicles (EVs) using electrochemical impedance spectroscopy (EIS). This system streamlines analysis, reducing time and cost for liquid biopsy diagnostics.

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    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Cancer Diagnostics

    Background:

    • Extracellular vesicles (EVs) are crucial biomarkers for early cancer detection.
    • Traditional EV isolation methods are time-consuming and complex.
    • Need for rapid, cost-effective diagnostic tools for liquid biopsies.

    Purpose of the Study:

    • To introduce the EV-Disrupt and Detect System (EDDS) for simultaneous detection of multiple EV biomarkers.
    • To enable swift, low-volume, and economical assessment of lung cancer biomarkers directly from serum.
    • To bypass conventional EV isolation techniques.

    Main Methods:

    • Development of a portable biosensor using electrochemical impedance spectroscopy (EIS).
    • Integration of electric field-induced EV disruption (50 mV, 1 kHz, 30s) with multiplexed biomarker detection.
    • Optimization of electric field parameters using 150 μL serum.
    • Validation of disruption efficiency (93.9%) and biomarker quantification against nanoparticle tracking analysis and ELISA.

    Main Results:

    • Achieved efficient EV disruption within 30 seconds, preserving protein integrity.
    • Optimized electric field parameters enhanced detectable biomarker concentrations by 0.218-2.809 fold.
    • Demonstrated simultaneous detection of TSG101, EGFR, GPC1, and GM2AP.
    • Corroborated EIS results with ELISA, confirming assay reliability.

    Conclusions:

    • The EDDS offers a streamlined, integrated platform for direct EV disruption and multiplexed biomarker detection.
    • This system significantly reduces processing time, cost, and complexity compared to traditional methods.
    • EDDS represents a robust tool for minimally invasive cancer diagnostics and advancing liquid biopsy applications.