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Engineering the efficient Exo-Cas12i2 for MITE manipulation in rice.

Nan Chai1, Wuge Liu2, Kangli Sun2

  • 1College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.

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|April 2, 2026
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Summary
This summary is machine-generated.

A novel enzyme fusion, Exo-Cas12i2 v1, precisely edits DNA in TA-rich regions and removes large genomic fragments. This technology aids in manipulating MITEs to enhance plant traits like root growth and cell elongation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • CRISPR-Cas systems are powerful genome editing tools.
  • Targeting specific DNA regions, especially GC-rich or AT-rich sequences, remains a challenge.
  • Understanding gene regulation in plant development is crucial for crop improvement.

Purpose of the Study:

  • To introduce a novel Cas12i2 variant (Exo-Cas12i2 v1) for efficient genome editing.
  • To demonstrate its capability in editing TA-rich regions and mediating large genomic deletions.
  • To explore its application in manipulating MITEs for agronomic trait enhancement.

Main Methods:

  • Construction and characterization of the Exo-Cas12i2 v1 fusion enzyme.
  • Assays to evaluate editing efficiency in TA-rich DNA sequences.
  • Application of Exo-Cas12i2 v1 for large genomic fragment deletion.
  • Exo-Cas12i2 v1-mediated MITE manipulation in plants.

Main Results:

  • Exo-Cas12i2 v1 successfully facilitated editing of TA-rich genomic regions.
  • The enzyme mediated deletions of large genomic fragments with high efficiency.
  • Exo-Cas12i2 v1-driven MITE manipulation precisely regulated genes involved in gibberellin-mediated cell elongation.
  • Targeted MITE manipulation influenced root ethylene responses.

Conclusions:

  • Exo-Cas12i2 v1 is a versatile genome editing tool effective in challenging DNA regions.
  • This technology offers precise control over gene regulation for improved plant development and agronomic traits.
  • Exo-Cas12i2 v1-mediated MITE manipulation presents a novel strategy for crop enhancement.