[Effect of Trifolium repens extract on OSC-4 cell proliferation, apoptosis and NLRP3/PRKD3 protein level]

To investigate the effects of Trifolium repens extract on proliferation, apoptosis, invasion and NLRP3/PRKD3 protein levels of oral squamous cell carcinoma cell line OSC-4.

The OSC-4 cells were randomly divided into SO group (OSC-4 cells were cultured without adding Trifolium repens extract), SA group (OSC-4 cells were cultured with 1 mg/mL Trifolium repens extract), SB group(OSC-4 cells were cultured with 5 mg/mL Trifolium repens extract), and SC group(OSC-4 cells were cultured with 10 mg/mL Trifolium repens extract). MTT was used to detect cell proliferation, flow cytometry was used to detect cell apoptosis, Transwsll chamber assay was used to evaluate cell invasion, Western blot and PCR were used to detect the expression of NLRP3/PRKD3.

Compared with SO group, the expression of NLRP3 and PRKD3 mRNA in SA group, SB group and SC group were significantly reduced, and the degree of reduction was in order of SC group＞SB group＞SA group(P＜0.05). There was no significant difference in the inhibition rate of cell proliferation in each group at 0 h(P＞0.05). At 48 h, the proliferation inhibition rate of SA group, SB group, and SC group was significantly higher than that of SO group(P＜0.05). There was significant difference in the apoptosis rate of OSC-4 cells in each group (P＜0.001). The apoptosis rate of OSC-4 cells in SA group, SB group and SC group was significantly higher than that in SO group(P＜0.05). There was significant difference in the number of OSC-4 cell invasion in each group(P＜0.001). The number of OSC-4 cell invasion in SA group, SB group and SC group was significantly lower than that in SO group(P＜0.05). The differences in the expression of NLRP3 and PRKD3 protein in each group was significant(P＜0.001). The NLRP3 and PRKD3 proteins of SA group, SB group and SC group were significantly lower than those of SO group (P＜0.05).

Sanye green extract can inhibit OSCC cell proliferation and invasion, promote its apoptosis. Its mechanism of action may be related to the regulation of NLRP3/PRKD3 expression.