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Flow Cytometric Characterization of Murine B Cell Development
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Multi-Parameter Spectral Flow Cytometry Panel for Immune Phenotyping of Murine B and T Cell Responses

Kassandra Hoetzel1,2, Hendrik Feuerstein1,2, Julia Ludwig1

  • 1B Cell Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

European Journal of Immunology
|April 3, 2026
PubMed

Keywords:
B cellsT cellsspectral flow cytometryvaccination

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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