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Evaluation of FilmArray Multiplex PCR Test Results in Bloodstream Infections in Oncological Patients
İpek Mumcuoğlu1, Sevgi Şahin1, Esra Tavukcu1
1Department of Clinical Microbiology, University of Health Sciences Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara, Turkey.
Background:
Rapid identification of microorganisms and resistance genes in positive blood cultures may help optimize early antibiotic therapy and improve outcomes. In this study, we compared the BioFire FilmArray Blood Culture Identification 2 (BCID2) panel results with routine microbiological examination methods in blood cultures that gave positive signals in an Oncology hospital.
Methods:
Gram stain results from positive blood culture bottles were reported to the clinic as panic value. After clinical evaluation, 93 positive blood cultures that met the inclusion criteria were evaluated with BCID2 panels in addition to routine microbiological examination. Microorganisms were identified at the species level with Vitek MS (MALDI-TOF, BioMérieux, France), and susceptibility tests were performed with the Vitek 2 (BioMérieux, France) system. BCID2 panel was compared with the routine microbiological methods.
Results:
Using BCID2 panels, microorganisms were identified in 87 out of 93 bottles. Growth occurred in the culture in 6 bottles that gave negative results with BCID2. These microorganisms are not included in the BCID2 panel. There was no growth in the culture in one bottle in which S. pneumoniae was detected by BCID2. While all bacteria that could be identified by BCID2 and Vitek MS were the same at the genus level, only 5 were different at the species level. Using BCID2, 59 bacteria from the samples included in the study were determined to carry one or more resistance genes.
Conclusion:
BCID2 panel provided faster results and its results showed a high correlation with traditional blood culture identification and antimicrobial sensitivity results.
