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Related Concept Videos

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing.

Enrique O Ruiz1,2, Kayla Neyra3, Diana M Lopez1,4,5

  • 1Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.

Biorxiv : the Preprint Server for Biology
|April 3, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a rapid method to isolate single-stranded DNA (ssDNA) scaffolds from double-stranded DNA (dsDNA) templates. This technique simplifies DNA origami nanodevice construction for diverse scientific applications.

Keywords:
DNA nanotechnologyDNA origamigene deliverynanoparticlessingle stranded DNA

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Area of Science:

  • Nanotechnology
  • Molecular Biology
  • Biophysics

Background:

  • Scaffolded DNA origami is a key nanoscale tool in biomedical and physical sciences.
  • Access to long single-stranded DNA (ssDNA) scaffolds is crucial for DNA origami nanodevices.
  • Current methods for ssDNA scaffold production are often inefficient and costly.

Purpose of the Study:

  • To develop a rapid and scalable method for isolating ssDNA scaffolds from double-stranded DNA (dsDNA).
  • To enable direct folding of DNA origami from dsDNA templates.
  • To expand the design possibilities for DNA origami nanostructures.

Main Methods:

  • Utilized blocking oligonucleotides to selectively release target ssDNA from dsDNA sources.
  • Applied the method to linear and supercoiled dsDNA of varying lengths (769–15,101 nucleotides).
  • Demonstrated a one-pot, thermally controlled reaction for direct DNA origami folding from dsDNA using blocking and staple strands.

Main Results:

  • Successfully isolated target ssDNA sequences from diverse dsDNA templates.
  • Achieved direct folding of DNA origami nanodevices from dsDNA templates in a single reaction.
  • Showcased the method's versatility with multi-scaffold and gene-encoding DNA origami structures.

Conclusions:

  • The developed method offers a faster, more efficient alternative for obtaining ssDNA scaffolds.
  • This approach simplifies DNA origami construction and broadens its applicability.
  • Facilitates the creation of advanced DNA origami structures for various scientific fields.