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An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore.

Sabrine Hedouin1, Changkun Hu1, Sue Biggins1

  • 1Basic Sciences Division, Howard Hughes Medical Institute, Fred Hutchinson Cancer Center, Seattle, WA 98109, United States.

Biorxiv : the Preprint Server for Biology
|April 3, 2026
PubMed
Summary
This summary is machine-generated.

The yeast transcription factor Cbf1 is crucial for chromosome segregation. It not only blocks centromeric transcription but also promotes inner kinetochore assembly, ensuring accurate chromosome distribution.

Keywords:
CCANCbf1centromerekinetochoremitosis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Kinetochore assembly on centromeric DNA is vital for chromosome segregation.
  • The protein Cbf1 binds centromeric DNA and its loss leads to chromosome missegregation.
  • Cbf1's role in preventing transcriptional readthrough was previously identified, but other functions remained unclear.

Purpose of the Study:

  • To elucidate the complete molecular mechanism of Cbf1 at the centromere.
  • To investigate Cbf1's role beyond transcriptional regulation in chromosome segregation.
  • To understand how Cbf1 contributes to kinetochore assembly and stability.

Main Methods:

  • In vitro and in vivo assays were used to study Cbf1's function.
  • Protein interaction studies were performed to identify binding partners of Cbf1.
  • Genetic analysis was conducted to assess the impact of Cbf1 on chromosome segregation.

Main Results:

  • Cbf1 was found to promote inner kinetochore assembly.
  • The interaction between Cbf1 and Okp1 is essential for this assembly function.
  • Cbf1's stable centromere association depends on its interaction with the inner kinetochore, highlighting interdependent roles.
  • Cbf1 acts as a hub, linking transcriptional control to kinetochore assembly and stability.

Conclusions:

  • Cbf1 has a dual role: transcriptional roadblocking and promoting kinetochore assembly.
  • Cbf1's interaction with Okp1 is critical for inner kinetochore formation.
  • An interdependent relationship between Cbf1 and the inner kinetochore ensures kinetochore stability and proper chromosome segregation.