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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Genome-based correction of misidentified MRSA: a case of multidrug-resistant Staphylococcus hominis
Chayanika Das1, Vinod Kumar Singh2, Vivek Kumar Singh3
1Division of Veterinary Microbiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122 India.
Abstract:
The present report communicates the genome sequence of a multidrug-resistant Staphylococcus hominis strain P1-NS, recovered from a nasal swab of a healthy pig in Bareilly, Uttar Pradesh, India. Initially misidentified as methicillin-resistant Staphylococcus aureus by phenotypic and PCR-based assays, whole genome sequencing clarified species identity as Staphylococcus hominis, emphasising the importance of genome-based diagnostics in antimicrobial resistance surveillance. The draft genome assembly consisted of 264 contigs of ≥ 200 bp with total genome assembly length of 2,199,316 bp, N50 of 137,700 bp, and GC content of 31.5%. A total of 2195 protein coding genes, 15 rRNA genes, and 70 tRNA genes, and multiple antimicrobial resistance determinants were present in draft genome assembly. This genomic information enhances understanding of antimicrobial resistance in livestock-associated coagulase-negative staphylococci in India.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13205-026-04766-3.
Insights
Whole genome sequencing identified a multidrug-resistant Staphylococcus hominis strain from Indian livestock, initially misidentified as Staphylococcus aureus. This highlights the critical role of genomic data in tracking antimicrobial resistance in animal populations.
Area of Science:
- Microbiology
- Genomics
- Veterinary Science
Background:
- Livestock can harbor multidrug-resistant bacteria, posing potential risks to public health.
- Accurate identification of bacterial species is crucial for effective antimicrobial resistance surveillance.
- Coagulase-negative staphylococci are increasingly recognized as important pathogens in both human and animal health.
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