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Comprehensive Bioinformatics Analysis and Experimental Verification RNF186 Is a Recurrence Signature Gene of

Shanbao Ke1, Junya Yan1, Xiao Feng1

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Summary

This study identifies key genes linked to hepatocellular carcinoma (HCC) recurrence using single-cell analysis. The developed prognostic model predicts patient survival and immunotherapy response, offering new therapeutic targets for HCC.

Keywords:
Hepatocellular carcinomaRNF186cell proliferationrecurrencesingle-cell RNA sequencing

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Area of Science:

  • Oncology
  • Genomics
  • Immunotherapy

Background:

  • Tumor recurrence significantly impacts prognosis in hepatocellular carcinoma (HCC).
  • The cellular and molecular drivers of HCC recurrence are not fully understood.
  • Identifying recurrence-associated genes is crucial for improving patient outcomes.

Purpose of the Study:

  • To identify genes associated with HCC recurrence at single-cell resolution.
  • To develop a prognostic model for predicting survival and immunotherapy response in HCC patients.
  • To explore the clinical significance of identified prognostic biomarkers.

Main Methods:

  • Integrated single-cell RNA sequencing data from primary and recurrent HCC samples.
  • Employed principal component analysis and t-SNE for cell clustering and gene identification.
  • Constructed a recurrence-related risk score using LASSO-Cox regression and developed a nomogram.
  • Assessed prognostic performance via Kaplan-Meier analysis and ROC curves, and analyzed immune infiltration.

Main Results:

  • The prognostic model accurately stratified HCC patients into high- and low-risk groups with distinct survival outcomes.
  • High-risk patients exhibited altered immune profiles and predicted reduced response to immunotherapy.
  • GRID2, RNF186, and SLC4A10 were identified as independent prognostic genes, with RNF186 promoting HCC cell proliferation.

Conclusions:

  • The developed prognostic model offers insights into precision medicine for HCC.
  • It highlights potential for predicting immunotherapy responsiveness in hepatocellular carcinoma.
  • RNF186 emerges as a potential therapeutic target for HCC treatment.