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Updated: Apr 4, 2026
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Analysis of Cell Migration within a Three-dimensional Collagen Matrix
Published on: October 5, 2014
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Cytokines IL-6, IL-10, and CCL5 Secreted by Infiltrating B Cells Promote Cell Migration of Human Prostate Cancer Cell
Crystal J Byrd1, Monasia Evans1, Woojung Kim2
1Department of Biological Sciences, Clark Atlanta University, Atlanta, GA 30314, USA.
Oncology Research
|April 3, 2026
Summary
Infiltrating B cells enhance prostate cancer cell migration and metastasis through secreted cytokines like IL-6, IL-10, and CCL5. Neutralizing these cytokines reduces cancer cell movement, suggesting a therapeutic target.
Area of Science:
- Oncology
- Immunology
- Cell Biology
Background:
- The tumor microenvironment influences prostate cancer metastasis.
- Infiltrating immune cells, including B cells, can play pro- or anti-tumorigenic roles.
- Preliminary data suggests higher B cell density near prostate cancer cells indicates promotion of cancer progression.
Purpose of the Study:
- To investigate the role of infiltrating B cells in enhancing human prostate cancer cell migration.
- To identify specific B cell factors that promote prostate cancer cell motility.
- To explore the impact of B cell cytokines on epithelial-mesenchymal transition in prostate cancer cells.
Main Methods:
- Transwell® assays were used to assess human prostate cancer cell migration.
- Evaluated migration in the presence/absence of B cells and B cell-secreted cytokines.
- Western blot and immunofluorescence staining analyzed epithelial-mesenchymal transition markers.
Main Results:
- Co-cultured B cells and specific cytokines (IL-6, IL-10, CCL5) increased prostate cancer cell migration.
- Neutralizing antibodies against these cytokines reduced B cell-induced migration.
- B cell cytokines elevated N-cadherin and Slug expression and disorganized ZO-1 in prostate cancer cells.
Conclusions:
- Infiltrating B cells enhance prostate cancer cell migration via secreted factors.
- These findings suggest a mechanism by which B cells contribute to prostate cancer metastasis.
- Targeting B cell-secreted cytokines may offer a strategy to inhibit prostate cancer progression.
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