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Updated: Apr 5, 2026
Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Application of polyoxometalate-based metal-organic framework modified magnetic black phosphorus nanosheets for
Dandan Jiang1, Lan Lan1, Yangyang Li1
1Inner Mongolia Engineering Research Centre of Lithium-Sulfur Battery Energy Storage, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Inner Mongolia Key Laboratory of Solid State Chemistry for Battery, College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao, 028000, China.
Abstract:
In this work, polyoxometalate-based metal-organic framework modified magnetic black phosphorus nanosheets (Fe3O4/BPNSs/POMOF) were designed and constructed. The resulting composite exhibited high surface area, excellent hydrophilicity, and superparamagnetism. Fe3O4/BPNSs/POMOF combined the Fe-O groups of Fe3O4, the metal oxide clusters of polyoxometalate, and rich Zn2+ ions along with inherent porosity of MOF. Fe3O4/BPNSs/POMOF was employed as a magnetic solid-phase extraction adsorbent for highly efficient phosphopeptide enrichment prior to mass spectrometry analysis. By integrating the principles of immobilized metal ion affinity chromatography and metal oxide affinity chromatography, Fe3O4/BPNSs/POMOF achieved high selectivity, enabling phosphopeptide capture even with a 5000-fold excess of competing peptides. The material had good recyclability (10 cycles) and high recovery (90.7 %) for phosphopeptides. The material maintained efficient enrichment capability for complex biological samples such as A549 cell lysate, highlighting its practical utility in phosphoproteomic research.
