Precise capture of heterogeneous microscale targets using an integrated spherical vortex acoustic tweezer

Acoustic micromanipulation provides a non‑contact approach for life science research, however, the precise manipulation of morphologically heterogeneous biological specimens remains a persistent challenge. Here, we report an integrated spherical vortex acoustic tweezer (ISVAT) operating at 40  MHz. The device is based on surface acoustic waves and incorporates a spiral interdigital transducer designed through wavefront modulation to produce a Bessel‑type spherical acoustic vortex, resulting in a compact, microscopy‑compatible three-dimensional potential well. Experimental results show that the ISVAT enables complex two‑dimensional trajectory control and selective separation of 20 μm polystyrene microspheres, exhibiting a lateral trapping force of approximately 262 pN. Importantly, the system achieves stable capture and controlled rotation of morphologically heterogeneous mouse sperm cells. This work demonstrates a high‑performance, integrated acoustic platform for the precise manipulation of diverse and irregular microscale targets, offering a versatile tool for single cell analysis and reproductive medicine research.