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Updated: Jul 13, 2026
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Published on: January 7, 2017
Rational MOF carrier engineering for tunable ATP detection
Jinfan Zhang1, Xiaopei Xie1, Hui Zhang1
1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Key Laboratory of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
This study introduces a novel carrier engineering method to tune adenosine triphosphate (ATP) detection sensitivity and linear range. By modifying carrier morphology and dosage, researchers achieved highly adaptable ATP assays with sub-picomolar detection limits.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biotechnology
Background:
- Adenosine triphosphate (ATP) is a vital biomarker for clinical diagnosis and microbial detection.
- Existing ATP assays require high sensitivity and tunable linear ranges due to biological sample variability.
- Zeolitic imidazole framework (ZIF)-mediated sensing offers a platform for ATP detection.
Purpose of the Study:
- To develop a carrier engineering approach for modulating ATP detection sensitivity and linear range.
- To investigate the impact of carrier morphology and dosage on ATP sensing performance.
- To establish a highly adaptable ATP detection method.
Main Methods:
- Preparation of micrometer-sized methylene blue (MB)-loaded ZIF-8 (MB@ZIF-8) and nanometer-sized MB@ZIF-8(CTAB) carriers with distinct morphologies.
- Modulation of probe encapsulation and carrier dosage to control sensing system parameters.
- Evaluation of sensing performance using fluorescence and photoelectrochemical readout modes.
Main Results:
- Carrier morphology engineering influenced MB loading capacity, with larger particles exhibiting higher loading.
- Adjusting carrier dosage allowed for regulation of the number of sensing units, broadening the linear range.
- Enhanced MB loading increased sensitivity, while increased carrier numbers expanded the dynamic range (4-6 orders of magnitude) with sub-picomolar detection limits.
Conclusions:
- Carrier engineering provides a versatile strategy for optimizing ATP sensing systems.
- The developed method enables system-level modulation of sensitivity and linear range for adaptable ATP detection.
- This approach offers a new pathway for developing highly sensitive and broadly applicable ATP assays.
