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The GW2-ERF115-SLRL2 module regulates seed dormancy in rice.

Jin-Dong Wang1, Li-Jun Kan1, Wu-Jian Shi1

  • 1Key Laboratory of Plant Functional Genomics of the Ministry of Education, Zhongshan Biological Breeding Laboratory, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, College of Agriculture, Yangzhou University, Yangzhou, Jiangsu 225009, China.

Journal of Genetics and Genomics = Yi Chuan Xue Bao
|April 4, 2026
PubMed
Summary

Researchers identified a new gene, ERF115, that helps rice resist pre-harvest sprouting (PHS). This discovery provides valuable genetic resources for breeding PHS-resistant rice varieties, improving crop yield and quality.

Keywords:
ERF115GW2PHSRice (Oryza sativa)SLRL2Seed dormancy

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Agricultural science

Background:

  • Pre-harvest sprouting (PHS) causes significant crop yield and quality losses.
  • Developing PHS-resistant rice is crucial but limited by genetic resources.
  • Ethylene response factor115 (ERF115) is a potential target for enhancing PHS resistance.

Purpose of the Study:

  • Identify genetic factors regulating seed dormancy and PHS resistance in rice.
  • Elucidate the molecular mechanism underlying ERF115's role in PHS resistance.
  • Discover elite alleles for breeding PHS-resistant rice cultivars.

Main Methods:

  • Screening of a rice mutant library to identify PHS-resistant mutants.
  • Genetic analysis to determine the function of ERF115.
  • Protein-protein interaction assays (ubiquitination, yeast two-hybrid).
  • Gene expression analysis and haplotype analysis.

Main Results:

  • The erf115 mutant showed enhanced PHS resistance, indicating ERF115 is a negative regulator of seed dormancy.
  • GW2 ubiquitinates ERF115, promoting its degradation and increasing PHS susceptibility.
  • ERF115 interacts with SLRL2, repressing MFT2 expression and reducing dormancy.
  • ERF115HapI is an elite allele associated with reduced PHS.

Conclusions:

  • A novel GW2-ERF115-SLRL2 regulatory module controls rice seed dormancy and PHS resistance.
  • This module integrates ubiquitin-mediated degradation and hormone signaling pathways.
  • Findings provide valuable genetic resources for breeding PHS-resistant rice varieties.