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A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
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Spatial Control in Covalent Multi-Enzyme Complexes Enables Efficient and Selective Redox Biocatalysis.

Zhi-Yong Du1,2,3,4, Xiao-Xiao Gong1,2,3,4, Feng-Qin Sun1,2,3,4

  • 1State Key Laboratory of Green Chemical Synthesis and Conversion, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

Journal of Agricultural and Food Chemistry
|April 4, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed covalently bonded multienzyme complexes (CBMEs) for efficient biocatalysis. This sustainable strategy enhances enzyme efficiency and selectivity in redox biosynthesis, reducing waste and coenzyme use.

Keywords:
double–enzyme complexreaction-diffusion effectsredox biosynthesisspatial arrangement

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Area of Science:

  • Biocatalysis and Enzyme Engineering
  • Synthetic Biology
  • Green Chemistry

Background:

  • Coenzyme regeneration, efficiency, and selectivity are key challenges in designing biocatalytic cascades.
  • Developing sustainable and efficient methods for redox biosynthesis is crucial for green chemistry applications.

Purpose of the Study:

  • To present a sustainable strategy for constructing covalently bonded multienzyme complexes (CBMEs).
  • To enable efficient redox biosynthesis under mild and environmentally friendly conditions.
  • To develop a quantitative model for predicting enzyme complex performance.

Main Methods:

  • Construction of covalently bonded multienzyme complexes (CBMEs).
  • Development of a quantitative reaction-diffusion model incorporating a G factor to predict adaptation efficiency (ε).
  • Application of the model to design a dual-enzyme complex for L-amino acid biosynthesis.

Main Results:

  • The developed model quantitatively predicts the adaptation efficiency of dual-enzyme systems.
  • A dual-enzyme complex achieved over 99% conversion within 2 hours and >99.9% enantiomeric excess.
  • CBMEs significantly reduced coenzyme usage and reaction waste compared to traditional methods.

Conclusions:

  • Controlling enzyme spatial arrangement via covalent coupling enhances catalytic efficiency and selectivity.
  • CBMEs offer a green and sustainable framework for designing high-performance bioredox systems.
  • This approach addresses critical challenges in biocatalysis for industrial applications.