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Published on: September 15, 2023
In Silico Multi-omics Analyses of Kinesin Family Member 15 in Pan-Cancer and Validation in Tissue Microarray
Mengqin Xiang1, Qing Hu1, Yanchun Li1
1Department of Pathology, Hunan Provincial People's Hospital, Hunan Normal University, Changsha, China.
Introduction:
Kinesin family member 15 (KIF15) is a molecular motor protein that participates in bipolar spindle assembly and centrosome separation during metaphase in normal cells. However, accumulating evidence has shown that KIF15 is aberrantly regulated in the state of malignancy and is associated with the progression of cancers.
Materials And Methods:
We analyzed the mRNA expression, protein expression, single-cell gene expression, prognoses, and immune reactivities of KIF15 using the data from various sources, including the Cancer Cell Line Encyclopedia, The Cancer Genome Atlas database, the Clinical Proteomic Tumor Analysis Consortium Confirmatory/Discovery database, and Cancer Single-cell Expression Map. Additionally, immunohistochemistry was performed to validate KIF15 protein expression in human tumor samples.
Results:
The data from the present pan-cancer analysis elucidated that KIF15 expression was increased in more than 25 human tumors. Furthermore, it was found to be highly corre-lated with biological pathways, immune cell infiltration, and poor prognoses across multiple cancers. Additionally, tissue microarray analysis confirmed abundant cytoplasmic KIF15 expression in ovarian serous carcinoma and colorectal adenocarcinoma, with significantly higher levels compared to adjacent normal tissues.
Discussion:
Consistent patterns of high KIF15 expression were also evident across various cancer cell lines in the CCLE dataset. Collectively, these observations suggest KIF15 is in-timately involved in the progression of multiple human cancers. By analyzing both the pro-teins that interact with KIF15 and the genes that show correlation with its expression, KEGG pathway enrichment indicated that processes such as cell cycle regulation, gap and tight junction dynamics, involvement of microRNAs in cancer, and the p53 signaling pathway may underlie the role of KIF15 in cancer development.
Conclusion:
KIF15 may serve as a potential biomarker and oncogenic factor in multiple cancer types.
