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Updated: Jul 15, 2026
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Response surface-optimized QuEChERS-UPLC-MS/MS method for multi-antibiotic determination and dietary risk assessment
Rong Bao1, Rongting Zhuo2, Zhuxiang Zhong2
1Department of Physical and Chemical Analysis, Fujian Provincial Key Laboratory of Zoonosist Research, Fujian Center for Disease Control and Prevention, Fuzhou, China; Public Health School, Fujian Medical University, Fuzhou, China.
Abstract:
Antibiotic residues in animal-derived foods raise food safety concerns. This study developed a QuEChERS-UPLC-MS/MS method for the simultaneous detection of multiple antibiotics in animal-derived foods. Key QuEChERS pretreatment parameters were systematically optimized using response surface methodology with a central composite design to identify the optimal extraction solvents, salting-out agents, and clean-up sorbents. Method validation met the requirements of European Commission Implementing Regulation (EU) 2021/808, confirming suitability for rapid determination of 85 antibiotics across diverse food matrices. Analysis of 590 samples detected antibiotic residues in 39.8% of samples, representing 36 different antibiotics. Health risk assessment showed a Hazard Index (HI) < 1, indicating low risk at current consumption levels for the local population. Nevertheless, this high detection frequency highlights the need for continued monitoring of antibiotic residues in animal-derived foods.
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