Related Experiment Video
Updated: Apr 6, 2026
Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
Establishment of autoclave conditions for disaccharide-containing liquid media using spectrophotometry and
Kozue Shimabukuro1, Taito Kobayashi2, Eiji Yokoyama3
1Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan.
Abstract:
Disaccharides undergo heat-induced hydrolysis, generating monosaccharides. In Japan, 10% sucrose solution autoclaved at 121 °C for 10 min is commonly added to basal media, but this caused false-positive fermentation for Shigella spp. Spectrophotometry and high-performance liquid chromatography showed that autoclaving at 105 °C for 10 min suppressed hydrolysis and prevented false positives.
