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Updated: Apr 6, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Navigating Blastocystis research - challenges in the next-generation sequencing era
Eleni Gentekaki1, Supaluk Popruk2, Pauline D Scanlan3
1Department of Veterinary Medicine, University of Nicosia School of Veterinary Medicine, Nicosia, Cyprus.
Abstract:
Blastocystis research has grown rapidly with molecular tools, especially next-generation sequencing, increasing subtype discovery but raising methodological and taxonomic challenges. We assess subtype designation criteria, caution against lowering divergence thresholds without biological evidence, and propose minimum standards requiring near full-length small subunit ribosomal RNA data and replication to ensure nomenclatural stability and epidemiological comparability.
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