Preparation of Pt-TiS₂ nanocomposite based amperometric glucose biosensor

In this study, an amperometric biosensor was developed using a glucose oxidase-functionalized Pt-TiS₂ nanocomposite for the sensitive and accurate detection of glucose. Pt nanoparticles (5-10 nm) were uniformly distributed on layered TiS₂ nanosheets (~100 nm length), ensuring high surface area and efficient electron transfer. The biosensor exhibited a linear detection range of 0.01-1 mM with a low detection limit of 0.018 μM and a quantification limit of 0.048 μM. The calculated sensitivity was determined to be 5.7392 μA·mM^-1·cm^-2, and the apparent Michaelis-Menten constant (K_m) was 0.138 mM, indicating strong enzyme-substrate affinity. The biosensor demonstrated exceptional selectivity against prevalent interferents, exhibiting high reproducibility (RSDs ranging from 0.20% to 4.20%) and notable reusability, with a mere 14.49% signal loss over the span of 10 cycles. The Pt-TiS₂/GOx biosensor exhibited 74.8% of its activity after a 60-day storage period at a temperature of 4 °C. In commercial serum samples, recovery rates were 106.9%, 105.7%, and 102.2% for 500, 1000, and 5000 μM glucose, respectively. The results obtained demonstrate the considerable potential of the Pt-TiS₂/GOx biosensor for accurate and reliable glucose monitoring in clinical settings.