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Freeze-Dried-Conserved Bacteria Can Be Identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass
Mennat El Ghalid1, Gérald Touak1, Dorian Garnier1
1Institut Pasteur, Biological Resource Center of the Institut Pasteur, Université Paris Cité, Paris, France.
Introduction:
Biobanking practices are continuously evolving to improve and accelerate sample preparation and analysis.
Objectives:
At the Biological Resource Center of the Institut Pasteur, our goal is to develop new and optimized methods for sample processing. In this study, we aimed to establish a culture-independent approach for identifying bacteria directly from their freeze-dried state.
Methods:
Freeze-dried bacteria from 25 species were reconstituted in saline solution, mechanically lysed, and their proteins extracted with acetonitrile and formic acid, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis.
Results:
The method enabled the reliable identification of diverse bacterial species. Spectrophotometric measurements led to the definition of a reproducible O.D.600 threshold, above which all strains were consistently and accurately identified.
Conclusion:
This approach provides a fast and robust alternative for the characterization of bacterial samples across diverse taxonomic groups.
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