Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Principles of Disease Surveillance

Principles of Disease Surveillance

Disease surveillance is the systematic collection, analysis, and interpretation of health data essential to the planning, implementation, and evaluation of public health practice. This process integrates data dissemination to entities responsible for preventing and controlling disease, injury, and disability. Surveillance systems provide crucial information for action, helping public health authorities make informed decisions to manage and prevent outbreaks, ensure public safety, optimize...

Investigation of Disease Outbreaks

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation.

Science translational medicine·2022

Same author

Lesion-attention pyramid network for diabetic retinopathy grading.

Artificial intelligence in medicine·2022

Same author

Development and Validation of a Nomogram to Predict Cancer-Specific Survival for Middle-Aged Patients With Early-Stage Hepatocellular Carcinoma.

Frontiers in public health·2022

Same author

Expanding the DNA-encoded library toolbox: identifying small molecules targeting RNA.

Nucleic acids research·2022

Same author

Depression duration and risk of incident cardiovascular disease: A population-based six-year cohort study.

Journal of affective disorders·2022

Same author

Adverse Events Reporting of Clinical Trials in Exercise Oncology Research (ADVANCE): Protocol for a Scoping Review.

Frontiers in oncology·2022

Search research articles

Related Experiment Video

Updated: Apr 7, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

Advances and applications in sequencing-based pathogen surveillance.

Hao Luo¹, Yao Wang¹, Huiyu Hou¹

¹Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

|April 6, 2026

Summary

Advanced sequencing technologies, including multiplex PCR and AI, offer improved pathogen detection. Innovative primer design and culturomics enhance sensitivity and specificity for global health applications.

Keywords:

Artificial intelligence Diagnostic methodologies High-throughput sequencing Pathogen detection

More Related Videos

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Related Experiment Videos

Last Updated: Apr 7, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Emerging infectious diseases require advanced diagnostic tools.
Traditional methods face limitations in speed, sensitivity, and scope.
High-throughput sequencing, especially multiplex PCR, shows promise for pathogen detection.

Purpose of the Study:

To review advancements in sequencing technologies for pathogen detection.
To highlight innovative primer design strategies for multiplex PCR.
To explore the integration of AI in sequencing for diagnostics.

Main Methods:

Culturomics, metagenomics, and metatranscriptomics for pathogen discovery.
Development of error-tolerant primer design balancing coverage and specificity.
Integration of artificial intelligence to enhance sequencing precision and scalability.

Main Results:

Multiplex PCR offers enhanced sensitivity, specificity, and cost-efficiency in pathogen detection.
Innovative primer design overcomes limitations like dimerization and bias.
AI integration enables real-time diagnostics and scalable sequencing.

Conclusions:

Advances in sequencing technologies and AI provide robust pathogen-detection systems.
These innovations support global health, food security, and ecological resilience.
Optimized multiplex PCR and AI integration are key to future diagnostics.