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Related Concept Videos

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Updated: Apr 8, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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CapSense-Flex: A Self-Powered Capillary Lab-on-Chip for Universal Electrochemical Biosensing.

Kirankumar Kuruvinashetti1,2,3, Bahareh Babamiri2,3, Azam Zare2,3

  • 1Intelligent Human Assisted Devices Group, University of Calgary, Calgary, Alberta T2N 1N4, Canada.

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|April 6, 2026
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Summary

CapSense-Flex is a novel capillary microfluidic chip for pump-free diagnostics. This wash-free platform enables precise molecular sensing with programmable incubation, ideal for point-of-care applications.

Keywords:
capillary microfluidicselectrochemical impedance spectroscopymolecularly imprinted polymerspoint-of-care biosensingwearable diagnostics

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Area of Science:

  • Microfluidics
  • Biosensing
  • Point-of-Care Diagnostics

Background:

  • Existing capillary microfluidic platforms for point-of-care (POC) diagnostics are limited by continuous flow, short residence times, and wash cycles.
  • These limitations hinder integration with affinity-based assays, restricting their diagnostic capabilities.

Purpose of the Study:

  • To introduce CapSense-Flex, a novel capillary chip for wash-free, diffusion-dominated molecular sensing.
  • To demonstrate its capability for programmable incubation windows (6-30 min) suitable for molecularly imprinted polymer (MIP)-based electrochemical detection.
  • To establish a universal architecture for scalable MIP-based biosensing in decentralized diagnostic formats.

Main Methods:

  • Laser-cut, roll-to-roll manufacturable capillary chip enabling autonomous fluid transport (≤25 μL) of various biofluids without pumps or valves.
  • Integration of a cortisol-selective MIP electrode for electrochemical detection.
  • Fiber-assisted wicking to prevent bubble formation and ensure accurate fluid-front arrival times (<5% deviation from predictions).

Main Results:

  • Log-linear quantification of cortisol in buffer and saliva (1-1000 ng mL⁻¹) with high linearity (R² = 0.9835) and a low limit of detection (0.1 ng mL⁻¹).
  • Demonstrated sensor stability with ~90% signal retention after 60 days at 4 °C.
  • Accurate quantification of cortisol in unprocessed saliva samples with minimal signal loss (<12%) compared to standards.

Conclusions:

  • CapSense-Flex offers a scalable, cost-effective (<$0.40/chip) platform for wash-free biosensing, decoupling transport from binding kinetics.
  • The plug-and-replace electrode design allows adaptation for various MIP targets.
  • This technology enables advanced MIP-based electrochemical detection for handheld, wearable, and decentralized diagnostic applications.