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Related Experiment Video

Updated: Apr 8, 2026

Reusable Hepatic Imaging Tool: A Reusable 3D-Printed Tool for Hepatic Intravital Microscopy
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Reusable Hepatic Imaging Tool: A Reusable 3D-Printed Tool for Hepatic Intravital Microscopy.

Mercedes B Cornelius-Muwanguzi1, Henry E Sanchez Cornejo2, Crispin H W Barnes3

  • 1Cavendish Laboratory, Department of Physics, University of Cambridge; Thoracic and GI Malignancies Branch, Center for Cancer Research, National Cancer Institute.

Journal of Visualized Experiments : Jove
|April 6, 2026
PubMed
Summary

A new 3D-printed device, the RHIT, enhances stability for liver intravital microscopy (IVM) in animal models. This innovation improves visualization of liver function and dysfunction, aiding research in metabolic diseases.

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Area of Science:

  • * Biomedical Engineering
  • * Cell Biology
  • * Medical Imaging

Background:

  • * Hepatic function and dysfunction are assessed using confocal-based intravital microscopy (IVM).
  • * Challenges in IVM include maintaining physiological conditions, stability, and repeatability, especially in animal models with increased weight and hepatic lipid content (e.g., Type II diabetes, MAFLD).

Purpose of the Study:

  • * To develop a novel imaging instrument to overcome constraints in hepatic IVM.
  • * To improve tissue access, stability, and reliability for dynamic liver visualization.

Main Methods:

  • * Design and fabrication of RHIT (a reusable, scalable, 3D-printed hepatic imaging device).
  • * RHIT allows for adjustable dimensions to fit different mouse sizes.
  • * Utilizes a brief surgical preparation to minimize animal handling, stress, and variability.

Main Results:

  • * RHIT demonstrates significant improvements in the stability and reliability of hepatic IVM.
  • * Enables detailed visualization of subcellular structures, including lipid droplets.
  • * Facilitates clearer, more reproducible metabolic and physiological studies, including longitudinal designs.

Conclusions:

  • * RHIT offers a standardized, accessible solution to long-standing technical challenges in hepatic IVM.
  • * The device improves the precision and reproducibility of liver imaging.
  • * Supports advanced research in liver disease and metabolism.