Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.4K
Crossing Over01:30

Crossing Over

7.3K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
7.3K
Homologous Recombination02:31

Homologous Recombination

65.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.7K
Homologous Recombination02:31

Homologous Recombination

7.4K
7.4K
Gene Conversion02:08

Gene Conversion

10.9K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.9K
Recombinant DNA01:09

Recombinant DNA

104.9K
Overview
104.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SbHsp70 overexpression enhances drought and salinity tolerance in wheat through improved cellular stability and stress-associated structural adaptations.

Frontiers in plant science·2026
Same author

Higher functional-longevity genomic merit is associated with lower morbidity, shorter calving interval, and greater herd retention in Holstein cows matched for milk-production merit.

Journal of dairy science·2026
Same authorSame journal

Genomic partitioning and functional dissection of inbreeding depression for stature in Brown Swiss cattle.

Genetics, selection, evolution : GSE·2026
Same author

Multifunctional SiO<sub>2</sub>&PB particles composite coating on a titanium surface enhances the osseointegration of dental implants in diabetes.

Colloids and surfaces. B, Biointerfaces·2026
Same author

A single-subject finite element study on the influence of root dislocation direction on inferior alveolar nerve stress during mesially impacted mandibular third molar extraction.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2026
Same author

An inherited SLC25A12-related recessive form of congenital porencephaly in Limousin cattle.

Genetics, selection, evolution : GSE·2026
Same journal

The mitogenome diversity of Alpine Rendena cattle: new clues on its maternal origin and the complex substructure of haplogroup T3.

Genetics, selection, evolution : GSE·2026
Same journal

Modest contribution of metabolomic data to genomic prediction of breeding values for feed conversion ratio in pigs.

Genetics, selection, evolution : GSE·2026
Same journal

Determining crossover count and position in two pig lines with different selection histories.

Genetics, selection, evolution : GSE·2026
Same journal

Effect of methylation on genome mutability in cattle.

Genetics, selection, evolution : GSE·2026
Same journal

Genomic selection strategies and their potential to maintain rare alleles and de-novo mutations: a long-term assessment.

Genetics, selection, evolution : GSE·2026
See all related articles

Related Experiment Video

Updated: Apr 8, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

9.9K

A comparative study on recombination activity in cattle.

Dörte Wittenburg1, Nina Melzer2, Nayyer Abdollahi Sisi2

  • 1Research Institute for Farm Animal Biology (FBN), 18196, Dummerstorf, Germany. wittenburg@fbn-dummerstorf.de.

Genetics, Selection, Evolution : GSE
|April 6, 2026
PubMed
Summary
This summary is machine-generated.

Breed-specific cattle genetic maps reveal distinct male and female recombination patterns, aiding genomic evaluations and breeding strategies. Female maps differ by breed purpose, while male maps show breed-specific clustering.

More Related Videos

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.9K
Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

1.0K

Related Experiment Videos

Last Updated: Apr 8, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

9.9K
Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.9K
Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

1.0K

Area of Science:

  • Animal Genetics
  • Genomics
  • Bioinformatics

Background:

  • Genetic diversity in cattle breeds necessitates breed-specific genetic maps for enhanced breeding strategies.
  • Understanding demographic history and breeding objectives (meat, dairy, dual-purpose) is key.

Purpose of the Study:

  • To derive breed-specific genetic maps (linkage maps) for commercial cattle breeds.
  • To analyze recombination patterns and their relationship with breed characteristics.

Main Methods:

  • Analysis of genotype data from six cattle breeds (4,181–76,875 samples).
  • Streamlined data preparation to handle missing data from various genotyping assays.
  • Application of three analytical approaches to derive genetic map coordinates for ~50K SNP markers.

Main Results:

  • Male map lengths ranged from 26.97–29.83 M, female map lengths from 23.32–26.08 M.
  • Female recombination activity and genetic maps were distinct from males.
  • Male maps clustered by breed (e.g., Brown Swiss, Simmental, Angus), while female maps clustered by breed purpose (dairy vs. dual-purpose).
  • Genome-wide association study identified chromosomal regions (BTA6, BTA10) linked to recombination activity.

Conclusions:

  • Breed-specific female genetic maps clearly differentiate from male maps.
  • Female maps cluster by breed purpose, unlike male maps.
  • Updated CLARITY app (v3.0.0) incorporates these findings for improved genomic evaluations and novel breeding strategies.